Semaglutide Dosing Guide:
Protocol, Reconstitution & Tracking

Complete evidence-based Semaglutide dosing protocol for 2026. Updated reconstitution steps, injection guidance, and cycle protocols.

⚠️ Medical Disclaimer: This content is for informational purposes only and does not constitute medical advice. Always consult a licensed healthcare provider before starting any peptide or medication protocol.

⚡ Semaglutide Quick Reference

Common dose: 250–2,400 mcg  |  Frequency: Once weekly
Half-life: ~7 days  |  Category: GLP-1 / Weight Loss
Injection sites: Subcutaneous — abdomen, thigh, or upper arm

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What is Semaglutide?

Semaglutide is a GLP-1 receptor agonist FDA-approved for type 2 diabetes (Ozempic) and chronic weight management (Wegovy 2.4 mg/week). It works by mimicking GLP-1: increasing glucose-dependent insulin secretion, suppressing glucagon, slowing gastric emptying, and powerfully reducing appetite through brain receptors. Clinical trials showed average weight loss of 15% body weight over 68 weeks. Starting dose: 0.25 mg weekly, titrated every 4 weeks. Compounded semaglutide requires reconstitution with bacteriostatic water. Track your weekly doses and titration schedule with the DoseIQ app.

Benefits of Semaglutide

Dosing Protocols

Below are evidence-based Semaglutide dosing protocols for different experience levels and goals. Always start at the lowest effective dose and assess tolerance before increasing.

Weeks 1–4: 0.25 mg (250 mcg) weekly — tolerability.
Weeks 5–8: 0.5 mg (500 mcg) weekly.
Therapeutic Range: 1 mg → 1.7 mg → 2.4 mg max, 4 weeks each.

Reconstitution Guide

Proper reconstitution is critical for accurate dosing. Follow these steps carefully:

1
Add 2 mL BAC water to 5 mg vial = 2,500 mcg/mL
2
0.25 mg = 0.10 mL | 0.5 mg = 0.20 mL | 1 mg = 0.40 mL | 2 mg = 0.80 mL
3
Inject subcutaneous abdomen, thigh, or upper arm
4
Refrigerate up to 28–56 days

Dosing Reference Table

Use this table to determine your exact injection volume based on vial size and BAC water amount:

Vial SizeBAC WaterConcentrationLow Dose VolHigh Dose Vol
5 mg2 mL2,500 mcg/mL0.10 mL0.40 mL
5 mg1 mL5,000 mcg/mL0.05 mL0.20 mL
10 mg2 mL5,000 mcg/mL0.05 mL0.20 mL

Injection Site Guide

Recommended sites: Subcutaneous — abdomen, thigh, or upper arm

Rotate injection sites to prevent scar tissue buildup. Clean the injection site with an alcohol swab before injecting. Use a 27-29 gauge insulin syringe for subcutaneous injections. Pinch the skin, insert needle at 45-90°, and inject slowly.

Storage Guidelines

Lyophilized (powder) form: Refrigerate at 36–46°F (2–8°C). Protect from light. Most peptides remain stable for 1–2 years unreconstituted.

Reconstituted solution: Refrigerate immediately after mixing. Use within 4 weeks typically. Do not freeze reconstituted peptides — this may damage the peptide structure.

Frequently Asked Questions

Q: Maximum dose?
A: 2.4 mg weekly (Wegovy for weight). 2 mg weekly for diabetes (Ozempic).
Q: How long to work?
A: Appetite suppression within week 1; significant weight loss by weeks 4–8; maximum results at 52–68 weeks.
Q: Missed dose protocol?
A: Within 5 days: inject ASAP. More than 5 days late: skip and resume next scheduled dose.
Q: How to reduce nausea?
A: Smaller meals, avoid high-fat foods, stay hydrated, titrate slowly, consider anti-nausea meds if needed.
Q: Best injection sites?
A: Rotate weekly between abdomen (2 inches from navel), outer thigh, and upper arm.

Track Your Semaglutide Protocol with DoseIQ

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