AOD-9604 Dosing Guide:
Protocol, Reconstitution & Tracking

Complete evidence-based AOD-9604 dosing protocol for 2026. Updated reconstitution steps, injection guidance, and cycle protocols.

⚠️ Medical Disclaimer: This content is for informational purposes only and does not constitute medical advice. Always consult a licensed healthcare provider before starting any peptide or medication protocol.

⚡ AOD-9604 Quick Reference

Common dose: 300–500 mcg | Frequency: Once daily, morning fasted
Half-life: 2–3 hours | Category: Fat Loss
Injection sites: Subcutaneous, abdomen

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What is AOD-9604?

AOD-9604 is a modified fragment of HGH (amino acids 176-191) specifically selected for its fat-burning properties without insulin-disrupting effects. Originally developed by Monash University, it stimulates lipolysis and inhibits new fat formation through beta-3 adrenergic pathways. Unlike HGH, it doesn't affect IGF-1 or insulin sensitivity. Works best injected in a fasted morning state. Often stacked with GLP-1 agents for synergistic weight loss. Track your AOD-9604 fasting protocol with the DoseIQ app.

Benefits of AOD-9604

Directly stimulates lipolysis (fat breakdown)
Inhibits new fat cell formation
No effect on blood glucose or IGF-1
Safe metabolic profile
Best results in fasted state
May support cartilage repair

Dosing Protocols

Below are evidence-based AOD-9604 dosing protocols for different experience levels and goals. Always start at the lowest effective dose and assess tolerance before increasing.

Standalone Fat Loss: 300–500 mcg morning fasted. 3–6 months.

GLP-1 Stack: 300 mcg AOD-9604 AM + semaglutide/tirzepatide weekly.

Cutting Protocol: 500 mcg daily fasted with caloric deficit.

Reconstitution Guide

Proper reconstitution is critical for accurate dosing. Follow these steps carefully:

Add 2 mL BAC water to 2 mg vial = 1,000 mcg/mL

300 mcg = 0.30 mL | 500 mcg = 0.50 mL

Take fasted — wait 30–60 min before eating

Dosing Reference Table

Use this table to determine your exact injection volume based on vial size and BAC water amount:

Vial Size	BAC Water	Concentration	Low Dose Vol	High Dose Vol
2 mg	2 mL	1,000 mcg/mL	0.30 mL	0.50 mL
5 mg	2 mL	2,500 mcg/mL	0.12 mL	0.20 mL
2 mg	1 mL	2,000 mcg/mL	0.15 mL	0.25 mL

Injection Site Guide

Recommended sites: Subcutaneous, abdomen

Rotate injection sites to prevent scar tissue buildup. Clean the injection site with an alcohol swab before injecting. Use a 27-29 gauge insulin syringe for subcutaneous injections. Pinch the skin, insert needle at 45-90°, and inject slowly.

Storage Guidelines

Lyophilized (powder) form: Refrigerate at 36–46°F (2–8°C). Protect from light. Most peptides remain stable for 1–2 years unreconstituted.

Reconstituted solution: Refrigerate immediately after mixing. Use within 4 weeks typically. Do not freeze reconstituted peptides — this may damage the peptide structure.

Frequently Asked Questions

Q: What is AOD-9604?

A: Fat-regulating HGH fragment (AA 176-191) that stimulates lipolysis without growth-promoting effects.

Q: When to take it?

A: Morning in a fasted state. Don't eat 30–60 min after injection.

Q: Expected fat loss?

A: Modest but significant per studies. Best results with caloric deficit and exercise.

Q: Stack with semaglutide?

A: Yes — complementary mechanisms. AOD-9604 stimulates lipolysis; GLP-1 agents reduce appetite.

Q: Cycle length?

A: 3–6 months. Favorable safety profile allows longer-term use.

Track Your AOD-9604 Protocol with DoseIQ

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AOD-9604 Dosing Guide:Protocol, Reconstitution & Tracking