Complete evidence-based TB-500 dosing protocol for 2026. Updated reconstitution steps, injection guidance, and cycle protocols.
Common dose: 2,000–5,000 mcg | Frequency: 2x/week loading, weekly maintenance
Half-life: ~4 days | Category: Healing & Recovery
Injection sites: Subcutaneous or IM, any site — acts systemically
TB-500 is a synthetic fragment of Thymosin Beta-4, providing systemic healing throughout the entire body from any injection point. Unlike BPC-157's localized effects, TB-500 acts body-wide by promoting cell migration, reducing systemic inflammation, and accelerating tissue repair everywhere. Used for muscle tears, tendon injuries, ligament damage, and post-surgical recovery. Standard protocol: loading phase (2–2.5 mg twice weekly for 4–6 weeks) followed by maintenance (weekly). The DoseIQ app tracks your TB-500 loading and maintenance schedule automatically.
Below are evidence-based TB-500 dosing protocols for different experience levels and goals. Always start at the lowest effective dose and assess tolerance before increasing.
Proper reconstitution is critical for accurate dosing. Follow these steps carefully:
Use this table to determine your exact injection volume based on vial size and BAC water amount:
| Vial Size | BAC Water | Concentration | Low Dose Vol | High Dose Vol |
|---|---|---|---|---|
| 5 mg | 2 mL | 2,500 mcg/mL | 0.80 mL | 1.00 mL |
| 10 mg | 2 mL | 5,000 mcg/mL | 0.40 mL | 0.50 mL |
| 5 mg | 5 mL | 1,000 mcg/mL | 2.00 mL | 2.50 mL |
Recommended sites: Subcutaneous or IM, any site — acts systemically
Rotate injection sites to prevent scar tissue buildup. Clean the injection site with an alcohol swab before injecting. Use a 27-29 gauge insulin syringe for subcutaneous injections. Pinch the skin, insert needle at 45-90°, and inject slowly.
Lyophilized (powder) form: Refrigerate at 36–46°F (2–8°C). Protect from light. Most peptides remain stable for 1–2 years unreconstituted.
Reconstituted solution: Refrigerate immediately after mixing. Use within 4 weeks typically. Do not freeze reconstituted peptides — this may damage the peptide structure.
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